Structure, dynamics and immunogenicity of a catalytically inactive CXC chemokine-degrading protease SpyCEP from Streptococcus pyogenes
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چکیده
منابع مشابه
The CXC chemokine-degrading protease SpyCep of Streptococcus pyogenes promotes its uptake into endothelial cells.
Streptococcus pyogenes expresses the LPXTG motif-containing cell envelope serine protease SpyCep (also called ScpC, PrtS) that degrades and inactivates the major chemoattractant interleukin 8 (IL-8), thereby impairing host neutrophil recruitment. In this study, we identified a novel function of SpyCep: the ability to mediate uptake into primary human endothelial cells. SpyCep triggered its upta...
متن کاملChemokine-cleaving Streptococcus pyogenes protease SpyCEP is necessary and sufficient for bacterial dissemination within soft tissues and the respiratory tractmmi_7065
Prathiba Kurupati,1 Claire E. Turner,1 Ioanna Tziona,1 Richard A. Lawrenson,1 Faraz M. Alam,1 Mahrokh Nohadani,3 Gordon W. Stamp,3 Annelies S. Zinkernagel,4 Victor Nizet,4 Robert J. Edwards2 and Shiranee Sriskandan1* Department of Infectious Diseases and Immunity, Department of Experimental Medicine and Toxicology, Histopathology Department, Imperial College London, Hammersmith Hospital, Du Can...
متن کاملChemokine-cleaving Streptococcus pyogenes protease SpyCEP is necessary and sufficient for bacterial dissemination within soft tissues and the respiratory tract
SpyCEP is a Streptococcus pyogenes protease that cleaves CXCL8/IL-8 and its activity is associated with human invasive disease severity. We investigated the role of SpyCEP in S. pyogenes necrotizing fasciitis and respiratory tract infection in mice using isogenic strains differing only in SpyCEP expression. SpyCEP cleaved human CXCL1, 2, 6 and 8 plus murine CXCL1 and 2 at a structurally conserv...
متن کاملThe CXC chemokine MIG/CXCL9 is important in innate immunity against Streptococcus pyogenes.
Pharyngitis caused by Streptococcus pyogenes is one of the most common bacterial infections in humans and is also a starting point for invasive S. pyogenes infection. Here, we describe that tonsil fluid from patients with streptococcal pharyngitis contains high amounts of the interferon (IFN)-dependent CXC chemokine known as monokine induced by IFN- gamma (MIG)/CXCL9. Also in vitro, inflamed ph...
متن کاملStreptococcus pyogenes SpyCEP Influences Host-Pathogen Interactions during Infection in a Murine Air Pouch Model
Streptococcus pyogenes is a major human pathogen worldwide, responsible for both local and systemic infections. These bacteria express the subtilisin-like protease SpyCEP which cleaves human IL-8 and related chemokines. We show that localization of SpyCEP is growth-phase and strain dependent. Significant shedding was observed only in a strain naturally overexpressing SpyCEP, and shedding was no...
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ژورنال
عنوان ژورنال: Computational and Structural Biotechnology Journal
سال: 2020
ISSN: 2001-0370
DOI: 10.1016/j.csbj.2020.03.004